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Three single-element thin metal foils of known areal density (Mn 18.9 μg cm−2, Fe 50.1 μg cm−2 and Pt 42.2 μg cm−2, Micromatter, Canada) were used to calibrate the relationship between fluorescence flux at the detector and elemental abundance

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The morning is generally considered the best time because taking it on an empty stomach allows for the highest absorption rate